PAF Assay - From David Gillis

Dear All

I'd welcome any assistance that can be offered on availability of PAF (platelet activating factor) assay, required for a patient of David Gillis.

STAT3 Mutation in ?Hyper-IgE Syndrome - From Melanie Wong

Thanks to Melanie for informing us of availability of this assay at The Children's Hospital, Westmead, as well

STAT3 Mutation in ?Hyper-IgE Syndrome - From Prof YL Lau

Dear All,

We have been providing e-consultation on suspected PID patients for Asian pediatric colleagues and lately African colleagues as well. 

STAT3 Mutation in ?Hyper-IgE Syndrome - From Stuart Tangye

Thanks to Stuart Tangye for informing me of assay availability through his laboratory, as well as performance of genotyping through Matthew Cook's laboratory

STAT3 Mutation in ?Hyper-IgE Syndrome - From Rohan Ameratunga

Thanks to Rohan for his information on assay availability, through LabPlus:

"Available at LabPlus, Auckland Hospital.  Kathryn can contact Dr See-Tarn Woon for costs etc if she wishes to proceed.Regards Rohan Ameratunga "

VEGF Assay - From Margret Schuller

Dear All

Thanks to Margret for conveying details of one the commercially-available ELISA kits for measuring VEGF levels - in this case, from Peprotech.com

Details of Peprotech VEGF ELISA available here

VEGF Assay - From Gary Unglik

Dear All

I'd appreciate feedback from anyone with knowledge of any lab's performing assay for VEGF levels. Input welcome.

Some additional info:

STAT3 Mutation in ?Hyper-IgE Syndrome - From Kathryn Patchett

Dear All

I'd be grateful for any help that can be provided on availability of the STAT3 mutation analysis assay. The patient in question is a 15 y.o. man

Quorn Allergy - From Raymond Mullins

Dear Glenn,
I have a paediatric patient @ 10 years  who has had anaphylaxis to quorn in a vegetarian meal.

Specific in-vitro IgE Testing to Opiates - From Richard Steele, Kathryn Patchett

Dear All

As you can see, availability for this testing (by CAP) is quite widely available

Thanks to Kathryn & Richard for adding to the chorus:

Specific In-Vitro IgE Testing to Opiates – From Daman Langguth

Thanks to Daman for his further clarification on this topic:

Specific In-Vitro IgE Testing to Opiates – From Penny Fitzharris

I believe Wellington has the "morphine" sIgE suggestive of NMBA hypersensitivity.

Specific In-Vitro IgE Testing to Opiates – From Robert Puy

HNY to all,

IgE antibodies to morphine (generally available as Phadia RAST) bind its single substituted ammonium group. They also bind neuromuscular blocking drugs at their substituted ammonium ions. The function of the test is to detect such antibodies in the investigation of anaphylaxis during general anaesthesia. Morphine itself single binding site for IgE and it cannot therefore cross-link IgE on the mast cell surface. If the clinical problem is rash or hypotension with opiates, it is likely due to a direct effect of the opiate on the mast cell and detection of IgE to morphine or other opiate would not be useful. 

 

Robert Puy

Specific IgE to Opiates - From Chris Bundell

PathWest QEII, Western Australia has the morphine cap for the Immunocap 250,  but we don't however have caps for the others.

 

Regards

Chris Bundell  PhD (UWA)

Clinical Immunology

PathWest Laboratory Medicine

QEII Medical Centre

Locked Bag 2009

Nedlands

Western Australia  6009

 

Tel:       (08) 9346 1844

Fax:      (08) 9346 3160

Specific in-vitro IgE ("RAST") testing for opiates - From Myfanwy Spellerberg

Myfanwy passes on the following enquiry:

"We have had a request from a consultant anaesthetist to provide testing for Allergy to Morphine, Codeine and Fentanyl  ( all Opiates )

In a patient with a history of bad reactions to opiates who is scheduled for THJR  

I was hoping that a RAST or similar type  test would be available.

It would appear that it is not being done in New Zealand

It would be a great help if you could check with the Immunology websites and advise if It is available elsewhere in Australasia."

FceRI Antibodies - From William Smith

My understanding is that there are still no commercial tests, the area has been difficult for technical reasons and also for reasons of test peformance- poor specificity in immunoassays, poor correlation between immuno and functional assays. Functional assays (BAT and histamine release) remain gold standard but reproducibility is poor and difficult to commercialise.
Regards, William

Anti-FcERIa and anti-IgE

Hi Martyn,

I came across this from the Mayo Clinic: http://www.mayomedicallaboratories.com/test-catalog/print.php?unit_code=91788

It looks like the test is done by National Jewish, and they look at CD203c upregulation on donor basophils.

Has anyone here sent them serum before?

I have been testing for anti-FcERIa and anti-IgE by an in-house RIA for research. However, this has not been validated yet. 

Priscilla

Priscilla Auyeung 

PhD student
Harrison Lab
Walter and Eliza Hall Institute
1G Royal Parade, Parkville, VIC 3052, Australia
Tel: +61 3 9345 2459
Fax: +61 3 9345 2911
auyeung@wehi.edu.au

Autoantibodies to FceRI or IgE

Is anyone aware of the availability of assays for autoantibodies to FceR1a or IgE?

 

Martyn

 

Professor Martyn A French MB ChB, MD, FRCPath, FRCP, FRACP
Clinical Immunologist, Department of Clinical Immunology, Royal Perth Hospital and PathWest Laboratory Medicine
Winthrop Professor in Clinical Immunology and Deputy Head, School of Pathology and Laboratory Medicine, University of Western Australia
Telephone: +61-8-92242899
Fax: +61-8-92242920
E-mail: martyn.french@health.wa.gov.au (hospital); martyn.french@uwa.edu.au (university)