Anti-FcERIa and anti-IgE

Hi Martyn,

I came across this from the Mayo Clinic: http://www.mayomedicallaboratories.com/test-catalog/print.php?unit_code=91788

It looks like the test is done by National Jewish, and they look at CD203c upregulation on donor basophils.

Has anyone here sent them serum before?

I have been testing for anti-FcERIa and anti-IgE by an in-house RIA for research. However, this has not been validated yet. 

Priscilla

Priscilla Auyeung 

PhD student
Harrison Lab
Walter and Eliza Hall Institute
1G Royal Parade, Parkville, VIC 3052, Australia
Tel: +61 3 9345 2459
Fax: +61 3 9345 2911
auyeung@wehi.edu.au

Autoantibodies to FceRI or IgE

Is anyone aware of the availability of assays for autoantibodies to FceR1a or IgE?

 

Martyn

 

Professor Martyn A French MB ChB, MD, FRCPath, FRCP, FRACP
Clinical Immunologist, Department of Clinical Immunology, Royal Perth Hospital and PathWest Laboratory Medicine
Winthrop Professor in Clinical Immunology and Deputy Head, School of Pathology and Laboratory Medicine, University of Western Australia
Telephone: +61-8-92242899
Fax: +61-8-92242920
E-mail: martyn.french@health.wa.gov.au (hospital); martyn.french@uwa.edu.au (university)

GAD65 & GAD67

Dear Glenn,

 

The current literature on anti-GAD and Stiff-Person Syndrome and/or cerebellar ataxia doesn't discuss GAD67 any more. It is agreed that the antigen recognised is a different epitope on GAD65 to that recognised by diabetics without SPS (Solimena M and Carmilli PD TINS, 14(10) 452, 1991).

 

The majority of patients with SPS or cerebellar ataxia produce a "cobble-stone" immunofluorescence pattern in the granular layer on monkey cerebellum (see attached PDF). We screened around 20 diabetics with high levels of anti-GAD (RSR ELISA) with no evidence of neurological disease and none demonstrated the "cobble-stone" pattern. This suggests that the SPS sera recognize a fixation-dependent epitope on GAD65.

 

The presence of anti-amphiphysin IIF (and anti-amphiphysin blot positive) in patients with anti-GAD increases the likelihood of a tumour.

 

Regards

Bob Wilson

GAD65 & GAD67

Dear All

As you know the GAD antibody seen in Stiff-Person Syndrome is picked up on islet cell substrate (see picture below), even though this is usually used to detect the anti-GAD65 antibodies seen in DM Type I

Is anyone in our group aware of potential utility &/or availability of more specific antibody test for the GAD67 antigen which is more associated with the autoimmune condition affecting brain? Or are they so similar structurally that a cross-reactive antibody is adequate?

Any input welcomed

Regards

Glenn

Anti-GAD IIF Monkey Cerebellum:

Pancreas GAD Staining: